- Droplets of cell-free Xenopus cytoplasmic extract, showing microtubule structures in green (fluorescent tubulin).
- First divisions in a developing Xenopus laevis frog embryo [Anderson et al., Cell Reports 21 (2017)].
- Cycling cell-free Xenopus cytoplasmic extract in 500μm wide Teflon tubes (green: GFP-NLS indicating nuclei in a shared cytoplasm). Waves originate at the boundaries coordinating mitosis in space and time [Nolet et al., eLife 9, e52868, 2020].
- Our frogs are taken good care of in the aquatic facility.
The world is a complex and dynamic place. Earth takes part in an intricate dance with the moon, surrounding planets, our sun, other stars and entire galaxies. All interact with one another determining our position in the universe. On a much smaller scale, humans consist of trillions of cells that work together to let us walk, run, and think. Each such single living cell is driven by the interaction of about a trillion non-living molecules. Life at all scales is complex, dynamic, and difficult to understand. All these examples, however, have in common that they obey the basic laws of physics. Although one can apply those laws to understand a small part of each system, many interacting parts can behave wildly different and unpredictable.
Our lab seeks to gain a fundamental understanding of the dynamical processes that coordinate living systems by using an interdisciplinary approach combining experimental biology and theoretical physics. More specifically, we aim to create artificial cells displaying life-like behavior, both through in vitro experiments and in silico models.